Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue SamplesAlison M. Moore 1,2, Lee R. Boudreau 3, Shakeel Virk 3, David P. LeBrun 1,2
1Department of Pathology and Molecular Medicine, Queen's University, 2Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, 3Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University
We describe the use of quantitative immunoblotting to validate immunofluorescence histology coupled with image analysis as a means of quantifying a protein of interest in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Our results demonstrate the utility of immunofluorescence histology for ascertaining the relative quantity of biomarker proteins in routine biopsy samples.
